BIO230H1 Lecture Notes - Lecture 2: Repressor, Positive Feedback, Synthetic Biology
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Bio230 lec 3 prokaryotic transcriptional regulation sep 18. Topics: prokaryotic gene regulation (recap, bacteriophage lambda, synthetic biology, transcription attenuation. Ligand binds -> allow the regulatory protein to bind dna. When bound to dna, the repressor protein prevents transcription (negative regulation) With ligand, it removes the repressor, switching/turning the gene on to promote the transcription. With ligand removed, the repressor bounds to dna off. With ligand, the repressor binds to dna off. With the ligand removed, the repressor gets inactivated and removed from dna turning the gene on. When bound to dna, the activator protein promotes transcription (positive regulation) With ligand, the activator is removed off. With ligand removed, the activator bounds on. Not yet seen an example but still exists in nature. With the ligand removed, the activator gets inactivated and removed from dna and rna polymerase gene off: negative vs.
