BIO230H1 Lecture Notes - Lecture 3: Exon Junction Complex, Alternative Splicing, Polyadenylation

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BIO230H1 Lecture Notes - Lecture 2: Repressor, Positive Feedback, Synthetic Biology

BIO230H1 Lecture Notes - Lecture 3: Exon Junction Complex, Alternative Splicing, Polyadenylation

BIO230H1 Lecture Notes - Lecture 4: Proteasome, Creb-Binding Protein, Phosphatase

Document Summary

Bio230 lec 5&6 post-transcriptional regulation sep 25. Topics: rna processing, rna nuclear export, mrna quality control, mrna stability. Translation can take place at the same time as the transcription occurs. Dna is circular coupled b/c the mrna need to be exported into the cytosol first for the translation. What coupled with the transcription are: 5" capping, rna splicing, 3" polyadenylation. Introns contain only the non-coding sequences: both exons and introns are transcribed into rna, but rna splicing would occur to chop off the introns, utr are the parts that are not coding (or not translated). However, they are transcribed: the coding starts from the start codon aug and terminates at the stop codon. 1: spliceosome and rna bound together to make an enzyme complex that governs the rna splicing, exon junction complex (ejc) is bound to the site where the splicing takes place. In other words, the site ejc binds to is the place that the intron used to be.

Related Questions

1. The Pribnow Box is a

A. attenuator

B. enhancer

C. promoter

D. operator

2. Which of the following do not occur during post-transcriptional processing of RNA in eukaryotes?

A. the splicing of introns

B. addition of a poly-A at the 3' end

C. removal of introns

D. addition of a 7-methyl guanosine cap at the 5' end

3. In transcription, RNA polymerase binds at

A. a promoter

B. an operator

C. the oriC locus

D. an enhancer

4. The Central Dogma in genetics describes

A. the pattern of information flow in the cell

B. the pattern of chromosomal inheritance in populations

C. the role of mutations in disease

D. the role of promoters

5. Which of the following sequences of DNA could code for a stem-loop structure (only the template strand is shown)?

A. 3'--G-G-C-C-C-T-T-G-G-A-A-A-A-C-C-A-A-G-G-G-C-C--5'

B. 3'--G-G-C-C-C-T-T-G-G-A-A-A-A-G-G-T-T-C-C-C-G-G--5'

C. 3'--G-G-C-C-C-T-T-G-G-T-T-T-T-G-G-C-C-C-T-T-G-G--5'

D. none of the above

6. Removal of introns from RNA is by

A. primosomes

B. peptidases

C. galactosidases

D. spliceosomes

7. Which of the following represents the units for measuring the rate of sedimentation of a molecule/organelle in a sucrose gradient?

A. molecular weight

B. Svedberg units

C. map units

D. Daltons

8. During transcription, which strand is not read by the RNA polymerase?

A. coding strand

B. noncoding strand

C. template strand

D. terminator strand

9. The function of the rho protein is

A. to help terminate translation

B. to help RNA polymerase bind to the DNA

C. to help RNA polymerase find a promoter

D. to help terminate transcription

10. The unusual bases found in transfer RNAs

A. are added during transcription

B. are normal bases that have been modified after transcription

C. help the tRNAs to bind to DNA

D. ensure that the tRNA stays linear in solution

1 DNA sequences found in introns provide [A] amino acid sequence information.

[B] regulatory information.
[C] no known useful information.

[D] structure for the gene.
[E] alternative DNA splicing possibilities.

3. The TATA box is a(n)
[A] sequence close to the promoter region of many genes.
[B] square-shaped sequence.
[C] enhancer consensus sequence.
[D] activator sequence necessary for proper translation.
[E] None of the above

5. Regarding genes and gene expression, which of the following is not a difference between prokaryotes and eukaryotes
[A] Eukaryotic DNA does not contain a TATA box close to the initiation site as does prokaryotic DNA.

[B] In eukaryotes, RNA polymerase requires

'transcription factors'or initiation.

[C] Prokaryotic genes are often grouped together in

operons.

[D] In eukaryotes, the regulation of several genes at once requires common control elements in each of the genes.
[E] There is a diversity of eukaryotic polymerases that is reflected in the diversity of eukaryotic promoters.

10) In eukaryotic cells, a repressor

[A] is made of DNA.
[B] binds to the enhancer region to block transcription. [

C] is located both upstream and downstream from the promoter.
[D] binds to the operator to block RNA polymerase.

[E] binds to a silencer to reduce transcription rates.

Coordinated regulation of genes in eukaryotic cells [A] is the result of positioning the same regulatory sequence in front of each gene.
[B] results from all of those genes being under the control of one promoter.

[C] occurs because related genes all have the same

operons.
[D] occurs because enhancers cause DNA to bend.

BIO230H1 Lecture Notes - Lecture 3: Exon Junction Complex, Alternative Splicing, Polyadenylation

BIO230H1 Lecture Notes - Lecture 2: Repressor, Positive Feedback, Synthetic Biology

BIO230H1 Lecture Notes - Lecture 3: Exon Junction Complex, Alternative Splicing, Polyadenylation

BIO230H1 Lecture Notes - Lecture 4: Proteasome, Creb-Binding Protein, Phosphatase

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