BIO230H1 Lecture Notes - Microscopy, Fluorescein, Green Fluorescent Protein
42
BIO230H1 Full Course Notes
Verified Note
42 documents
Document Summary
Microscopy: techniques used to view live cells continues to be an invaluable tool provides images/insights into the mechanisms (ex. Biochemical processes: used to view cell signaling and cell-cell interaction. Learn how to adjust microscope (koehler illumination) Understand neglected concepts in optical microscopy with proper configuration. Intensity and wavelength spectrum of light emitted through various places. Organelles and other cytoplasmic components of cells are capable of movements. Plant cells part of the cytoplasm may show cytoplasmic streaming, which may result in broad cytoplasmic strands. Individual organelles, mitochondria and chloroplast, may position themselves within streaming or exhibit short periods of rapid movement. Recent studies show that in many cells, layer of cytoplasm and organelles close to plasma membrane. Nucleus commonly maintains its position in the cell regardless of cytoplasm movement. Fluorescence diacetate (fda): uncharged and non fluorescent derivative of a fluorescence molecule, fluorescein.
