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Lecture

Kelly Lec 3 2013 w note.pdf


Department
Biochemistry
Course Code
BCH311H1
Professor
Dr.Kelly

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Manipulation and molecular
characterization of nucleic acids
Reading nucleic acids sequences
! Restriction analysis
! Blotting
!Role of synthetic DNA
- DNA sequencing – Maxam-Gilbert, Sanger, etc
- PCR
Manipulating nucleic acids for the study of gene function
- Recombinant DNA technology
- Site directed mutagenesis
- Transgenic animals
- Transgenic plants
Other methodologies for studying nucleic acids
-microarrays
How to manipulate & sequence NA

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Decoding nucleic acids
How can genomes be
made into more
manageable fragments
for study?
How can base
sequences be read?
How can chromosomal
organization be studied?!
DNA
molecular
structure!
Genomic
structure!
Genomic sequence: governs function!
*We gotta break the structure to read!
-> systematic method is needed

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Single chromosome: packed!!!!! complicated!!!! how do we break this?
-> restriction nuclease (cut sequences at two sites (symmetric sites)
Limits: hard to determine the full sequence of a very large unknown DNA fragment..
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