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Class Notes for Biochemical Engineering and Applied Chemistry at University of Toronto St. George

BCH210H1 Lecture Notes - Lecture 8: Polyacrylamide, Toothpaste, Protein Structure
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BCH210H1(F) Lecture 8 SDSPAGE = sodium dodecyl sulfatepolyacrylamide gel electrophoresis o For analyzing protein samples works by separating proteins based on size of polypeptide o SDS is a very strong denaturing detergent...

Biochemical Engineering and Applied Chemistry
BCH210H1
Stavroula Andreopoulos, Roman Melnyk, Sian Patterson, Reinhart Reithmeier
BCH210H1 Lecture Notes - Lecture 11: Affinity Chromatography, High-Performance Liquid Chromatography, Elution

BCH210 Lecture 11 Analysis of proteins Purification by chromatography Amino acid composition Amino acid sequence Protein Purification Characterization of protein structure and function requires a pure sample Protein sampl...

Biochemical Engineering and Applied Chemistry
BCH210H1
Wright
BCH210H1 Lecture Notes - Lecture 8: Sodium Dodecyl Sulfate, Subtilisin, Fusion Protein

BCH210 - Lecture 8 SDS Gel Electrophoresis SDS - PAGE: Sodium dodecyl sulfate - polyacrylamide Proteins unfold and are separated by size SDS: Denaturing detergent and dis...

Biochemical Engineering and Applied Chemistry
BCH210H1
Wright
BCH210H1 Lecture Notes - Lecture 9: Protein Structure Prediction, X-Ray Crystallography, Globular Protein

BCH210 Lecture 9 Prediction of Protein Structure o o Anfinsen experiments shown that info for 2 and 3 structure is coded in the aa sequence Folding code the thermodynamic question of what balance of interatomic forces di...

Biochemical Engineering and Applied Chemistry
BCH210H1
Wright
BCH210H1 Lecture Notes - Lecture 10: Hemoglobin, Beta Helix, Beta Sheet

BCH210 Lecture 10 Hemoglobin A vs S Sickle cell anemia chronic hemolytic disease that is genetically transmitted HbA Reversible O2 carrier 4 subunits 2 alpha helix (141 aa each) 2 beta sheets (146 aa each) HbS differs fro...

Biochemical Engineering and Applied Chemistry
BCH210H1
Wright
BCH210H1 Lecture Notes - Lecture 7: Multiple Cloning Site, Recombinant Dna, Restriction Enzyme

BCH210 - Lecture 7 Genetic engineering: Molecule biology Change proteins by changing genes Proteins and their mutants can be obtained in quantities sufficient for research purp...

Biochemical Engineering and Applied Chemistry
BCH210H1
Wright
BCH210H1 Lecture Notes - Lecture 6: Alpha Helix, Pancreatic Ribonuclease, Ribonuclease

BCH210 Lecture 6 Protein folding Takes 103 to 101 sec to fold protein folding is direct Folding is not random, dedicate by the aa found in the linkage, Phi and psi angle restrict the way the proteins fold Early steps in p...

Biochemical Engineering and Applied Chemistry
BCH210H1
Wright
BCH210H1 Lecture Notes - Lecture 5: Globular Protein, Alpha Helix, Pancreatic Ribonuclease

BCH210 - Lecture 5 Fibers inside the cell For movement and structure Makes up the cytoskeleton Action Microfilaments Intermediate filaments Actin Filament Cytoskelet...

Biochemical Engineering and Applied Chemistry
BCH210H1
Wright
BCH210H1 Lecture Notes - Lecture 4: Side Chain, Alanine, Hydroxyproline

BCH210 Lecture 4 Trans conformation of the peptide (amide bond) H bond is about 1.3 A distance (very small) Resonance structures Gives the CN bond double bond characters The double bond properties restrict the rotation o...

Biochemical Engineering and Applied Chemistry
BCH210H1
Wright
BCH210H1 Lecture Notes - Lecture 12: X-Ray Crystallography, Fluorescence Spectroscopy, Diffractometer

BCH210 Lecture 12 Analysis of Protein structure Determining 3D structures: Xray crystallography Electron microscopy (EM) NMR Using wavelength to interact with proteins IR spectroscopy CD spectroscopy Fluorescence spectros...

Biochemical Engineering and Applied Chemistry
BCH210H1
Wright
BCH210H1 Lecture Notes - Lecture 3: Alpha Helix, Hydrogen Bond, Beta Sheet

BCH210 Lecture 3 4 levels of protein Primary (1) linear amino acid Secondary (2) periodic, repetitive units such as alpha helix, beta sheet, reverse turn Tertiary (3) the folding of 2 structure into defined protein s...

Biochemical Engineering and Applied Chemistry
BCH210H1
Wright
BCH210H1 Lecture Notes - Lecture 2: Aromatic Amino Acids, Cystine, Aspartic Acid

BCH210 - Lec 2 20 amino acids - Know Name Structures Properties pH: acid and basic Forces stabilizing protein structures Amino acids with Aliphatic side chains Glycine, Gly, G A...

Biochemical Engineering and Applied Chemistry
BCH210H1
Wright
BCH210H1 Lecture Notes - Lecture 1: Protein Folding, Sickle-Cell Disease, Red

BCH210 Lecture 1 Red Blood cells Hemoglobin Protein Red colour cause by the Fe heme complex Tetramer: 2 alpha and two beta polypeptide chains Sickle Cell Anemia Signal change in amino acid (beta change) Caused by the repl...

Biochemical Engineering and Applied Chemistry
BCH210H1
Wright
BCH210H1 Lecture Notes - Lecture 7: Multiple Cloning Site, Recombinant Dna, Antimicrobial Resistance
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BCH210H1(F) Lecture 7 Genetic engineering revolutionized the study of protein structure/function o Proteins can be modified by modifying their genes, meaning that if you want to look at the role of a pa...

Biochemical Engineering and Applied Chemistry
BCH210H1
Reinhart Reithmeier
BCH210H1 Lecture Notes - Lecture 5: Pancreatic Ribonuclease, Beta Sheet, Endoplasmic Reticulum
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BCH210H1(F) Lecture 5 3 main types of fibers make up the cytoskeleton (gives cell structure/shape and facilitates movement) inside the cell: Cytoskeleton ...

Biochemical Engineering and Applied Chemistry
BCH210H1
Reinhart Reithmeier
Inorganic Chemistry - Reference Guides

This Guide provides invaluable reference material to anyone who requires readily accessible information concerning the basic principles that underlie this complex science. The Guide carefully references the Periodic Table ...

Chemistry
CHEM 30100
All Professors
Permachart - Marketing Reference Guide: Iupac Nomenclature Of Inorganic Chemistry, Lithium Aluminium Hydride, Polyatomic Ion

p e r m a c h a rt s MT I n o r g a n i c C h e m i s t r y N o m e n c l a t u r e ...

Chemistry
CHEM 30100
All Professors
Permachart - Marketing Reference Guide: Locant, Parent Structure, Substituent

permacharts TM O r g a n icC h e m is t ryN o m e n c la t u r e Nam...

Chemistry
CHEM 22700
All Professors
Permachart - Marketing Reference Guide: Beta Particle, Beta Decay, Atomic Number

permacharts TM Periodic Table of the Elements ...

Chemistry
CHEM 11100
All Professors
Permachart - Marketing Reference Guide: Carnot Heat Engine, Carnot Cycle, Isentropic Process

permacha srtM PHYSICAL CHEMISTRY Basics Adiabatic process in which no heat is transferred into or out Internal energy The total kinetic and potent...

Chemistry
CHEM 11100
All Professors
Introductory Chemistry - Reference Guides

This Guide serves as a well organized and effective point of entry for anyone embarking upon the study of Chemistry. All fundamental terms are clearly organized in chart form, with illustrative graphics. More complex conce...

Chemistry
CHEM 11100
All Professors
Permachart - Marketing Reference Guide: Magnetic Circuit, Electrical Network, Angular Velocity

l e a r n • r e f e r e n c e • r e v i e w permacharts TM Electrical Engineering GLOSSARY OF TERMS ...

Electrical Engineering
4400:307
All Professors
Electricity & Magnetism - Reference Guides

This Guide is another one of the many products that effectively advances one’s knowledge of a distinct aspect of the physical sciences. The basic of the properties of electricity and electrical circuits are established in ...

Electrical Engineering
4400:307
All Professors
Permachart - Marketing Reference Guide: Semiconductor Device, Precision Rectifier, Envelope Detector

permacharts TM E le c t r o n ic sI Waveforms Diodes ...

Electrical Engineering
4400:307
All Professors
Permachart - Marketing Reference Guide: Schmitt Trigger, Square Wave, Negative Feedback

permacharts TM E le c t r o n ic sII Operational Amplifiers (OP AMPS) ...

Electrical Engineering
4400:307
All Professors

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