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BIOD29 4A.docx

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University of Toronto St. George
Aarti Ashok

BIOD29 Lecture 4A January 30, 2012 Pop Quiz Review You need to bind to the receptor before trypsin cleavage. If you cleave first, you won’t allow it the possibility to attach well to the Ace receptor. A receptor for Sars-Cov is Ace 2 and L sine. Trypsin is not a lysotropic drug. Trypsin cleaves the S protein into S1 and S2. Herpesviridae  Circularized once into human nucleus by ligating two linear strands together  Notice there are repeat elements: we haven’t discussed this yet  Tegument full of viral proteins and crucial part of viral infection  There are some levels of new strains arising but this is a DNA genome virus and DNA genome viruses tend not to evolve as much as RNA genome viruses Herpesviridae: Herpes simplex viruses  EBV is known as the kissing virus  CMV is not trouble to healthy individuals, but those who are not well such as those on immune depressant drugs  Tegument proteins are packaged inside the viral envelope and are introduced into the cell upon infection o Recent research says there may be even some viral mRNAs in this tegument portion  Still debating which envelope proteins are for receptor binding and which is for fusion  b’ is the flip of b, the same sequence is inverted  Herpes simplex viruses bind to heparin sulfate proteoglycans on the cell surface o heparin is linked to small protein fragments, it is the glycan part that is emphasized, a lot of glycan, a bit of protein o bind via envelope proteins, gB and gC o gB also binds PILR-alpha  PILR-alpha is the specific protein gB binds to  gD is more specifically binds and allows for the fusion to occur  Which proteins are the fusogenic proteins had not been nailed down as of yet Diagram  Mystery step is which envelope proteins help fuse the envelope to the plasma membrane Diagram  c. Fusion with PM  e. Nucleocapsids detach from tegument (arrowhead)  g. Empty capsids after release of DNA into nucleus o whatever was inside has been ejected  Dynein is a (-) end directed motor that is bringing things from cell periphery towards the cell nucleus Herpesviridae: Herpes simplex viruses  The immediate early genes were discovered after early genes hence the name  There is no use making structural proteins if you haven’t replicated your genome therefore gamma 2 genes are highly structural  Basal transcription, there is a low level of alpha gene expression initially, you need transactivator that will bind regulatory sequence of alpha gene it will tell the promoter to express a big deal and that something is a tegument protein  Vhs mediated the selective degradation of bother cellular and viral mRNA virion host shutoff protein o Have not evolved enough to make something that* o Going to rely on the fact that viral RNA accumulates faster than it is degraded, if you make a factor that is not specific but cleaves both cellular and viral mRNA then viral proteins predominate in the cell o Cellular mRNA are seriously inhibited o Viral proteins predominate in the cell  There is a nick made on purpose and replication is not bi directional replication, you start rolling circle mechanism  You nick one of the strands, the strand comes apart and you start with the other end of the strand.  You start copying the inner strand that is not nicked.  You are building your new DNA based on the template of the non-nicked
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