BIO130H1 Lecture Notes - Telomerase Rna Component, Phosphodiester Bond, Dna Replication
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BIO130H1 Full Course Notes
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Start codon: aug, encodes for euk: met, prok: fmet. Mutational steps: # of nucleotide changes to get to another codon. N-glycosidic bond: base nitrogen 9(pur) or 1(pyr) to 1" sugar carbon. Phosphodiester bond: 5" sugar carbon to 3" phosphate. Proteome: complete set of proteins in cell or organism. Interactome: complete set of interactions between proteins and molecules in cell and organism. 1 dsdna heat to denature-> 2 ssdna decrease temperature-> primers aneal to segment of interest heat to extend with taq polymerase-> thermostable polymerase extends strands, 2 dsdna repeat. Peptide bond: condensation rxn, alpha amino group to alpha carboxyl group, r" not involved. Types of aa: np, p uncharged, pcharged basic or. In chain, after condensation, amino turns to amide and carboxyl turns to carbonyl. Cysteine: can from disulphide bonds with in chain (creates hair pin structures), happens in oxidized situations (r"ch2sh, condensation reaction, ch2s-sch2) Order of aa"s are important, same chain reversed is chemically different.