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Lecture

BIO130Sec2Lecture1.pdf

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Department
Biology
Course
BIO130H1
Professor
Jane Mitchell
Semester
Winter

Description
Cellular Form and Function: The parts of a cell Bio 130 Part 2 Jennifer Mitchell 1 Bio130 Part 1 DNA mRNA mRNA protein 2 Membrane structure and transport Organelles Cytoskeleton Junctions, adhesion and ECM The cell cycle Figure 1-30 Molecular Biology of the Cell, Fifth Edition (© Garland Science 2008) 3 By the end of Bio 130 you should be able to: 1. Identify the different parts of a eukaryotic cell. 2. Match the processes that take place inside the cell with the parts of the cell. 3. Place these processes in context of how the whole cell functions. 4. Recognise the techniques used in different types of experiments discussed in lectures. 4 Asking questions about lecture material • Tutorial sessions after Thursday’s lecture. – Clicker questions. – Open Q & A. • Blackboard discussion board: – On Friday afternoon Prof Mitchell will respond to questions. 5 Saccharomyces cerevisiae 6 Multicellular organisms are composed of many different cell types 7 “Typical” Animal Cell 8 “Typical” Plant Cell • Nucleus • Endoplasmic reticulum • Golgi apparatus • Peroxisome • Mitochondria • Plasma membrane 9 Cell Picture Show, Image by Anne Weston, Cancer Research UK Membrane Structure Reading: Alberts et al. 5 edition Chapter 10, pages 617-624 and 629-640 10 1 2 3 1. Cytoplasm  2. Cytosol  3. Lumen  11 Many Cellular Functions Occur at Membranes 1. Compartmentalization 2. Scaffold for biochemical activities 3. Selectively H 0 permeable barrier 2 6. Interactions between cells 4. Transporting solutes 5. Responding to external signals 12 Cell Membranes - divides cell into compartments - control movement of molecules • lipid bilayer   • membrane proteins   The fluid mosaic model of the membrane 13 Lipid Bilayer Polar head groups } Hydrophobic tails Polar head groups  Amphiphilic: Leaflet   14 Lipid Bilayer continued • Phospholipids • polar head group • two hydrophobic tails Figure 10-2 • In an aqueous environment  Figure 10-7 15  Artificial lipid bilayers: Uses: 1) 2) 3) Cross-section view Figure 10-8 Molecular Biology of the Cell(© Garland Science 2008) 16 Lipid Components—Phospholipids 1) Phospholipids  • E.g. phosphatidylcholine, sphingomyelin - Hydrocarbon tails: (A)   Kink indicates that the tail is unsaturated, Contains a cis-double bond phosphatidylcholine 17 Lipid Components—Steroids 2) Steroids - animals: cholesterol - plants: plant steroids, some cholesterol - up t
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