BIO130H1 Lecture 8: Lecture 8 Part 2 (lec 9)

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peachzebra67

25 Mar 2012

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Transcription ii (pii) mrna creation from rna: 5" pre-mrna capping added to protects rna from exonucleases. Phosphatase removes a phosphate from 5" end: gmp is added by guano transferase, methyl group is added by methyl transferase to complete capping. Pre-mrna rna splicing: modification of rna after transcription to create mrna/prepare it for translation. Transcribed introns are removed (non-coding) and exons are joined together (coding: catalyzed by spliceosome, adenosine attacks 5" splicing site on exon, 5" of on exon reacts with 3" of other to release intron. Steps in ensuring correct translational unit pairing: trna synthetase (aminoacyl trna synthetase, makes sure correct trna has correct aa. Editing site cleaves off incorrect aa through hydrolytic editing: trna molecule, makes sure correct trna pairs with correct mrna codon. Located on cytosol (eukaryotic/prokaryotic: divided into, e-site: exit site, p-site: peptidyl site, elongation of aas, a-site: aminoacyl, where trna enters.

Related Questions

1. The Pribnow Box is a

A. attenuator

B. enhancer

C. promoter

D. operator

2. Which of the following do not occur during post-transcriptional processing of RNA in eukaryotes?

A. the splicing of introns

B. addition of a poly-A at the 3' end

C. removal of introns

D. addition of a 7-methyl guanosine cap at the 5' end

3. In transcription, RNA polymerase binds at

A. a promoter

B. an operator

C. the oriC locus

D. an enhancer

4. The Central Dogma in genetics describes

A. the pattern of information flow in the cell

B. the pattern of chromosomal inheritance in populations

C. the role of mutations in disease

D. the role of promoters

5. Which of the following sequences of DNA could code for a stem-loop structure (only the template strand is shown)?

A. 3'--G-G-C-C-C-T-T-G-G-A-A-A-A-C-C-A-A-G-G-G-C-C--5'

B. 3'--G-G-C-C-C-T-T-G-G-A-A-A-A-G-G-T-T-C-C-C-G-G--5'

C. 3'--G-G-C-C-C-T-T-G-G-T-T-T-T-G-G-C-C-C-T-T-G-G--5'

D. none of the above

6. Removal of introns from RNA is by

A. primosomes

B. peptidases

C. galactosidases

D. spliceosomes

7. Which of the following represents the units for measuring the rate of sedimentation of a molecule/organelle in a sucrose gradient?

A. molecular weight

B. Svedberg units

C. map units

D. Daltons

8. During transcription, which strand is not read by the RNA polymerase?

A. coding strand

B. noncoding strand

C. template strand

D. terminator strand

9. The function of the rho protein is

A. to help terminate translation

B. to help RNA polymerase bind to the DNA

C. to help RNA polymerase find a promoter

D. to help terminate transcription

10. The unusual bases found in transfer RNAs

A. are added during transcription

B. are normal bases that have been modified after transcription

C. help the tRNAs to bind to DNA

D. ensure that the tRNA stays linear in solution

11. What are the functions of the 5â cap? How is it added on the RNA?

12.Explain how a poly-A tail is produced; (CPSF and CstF, Poly APolymerase, Poly A Binding Protein (PABP)).

13.What are the two hypotheses for eukaryotic transcription termination?

14.How does splicing of pre-mRNA occur?

15.Compare and contrast splicing of group I introns, group II introns and spliceosome-mediated splicing of mRNA.

greylocust179

Promoters upstream of the gene are recognized by ____ in prokaryotes or ___ in eukaryotes. RNA polymerase transcribes RNA in a ____ direction. Termination is the result of the transcription of the RNA sequence signal, ____. Post-(or co-)transcriptional modifications include a 5' ____ and 3' ____ as well as splicing out ____. Spliceosomes consist of "snRNPs." SnRNP U1 binds the ___, snRNP U2 binds the ____, and snRNP U5 binds to the ____. In the first splicing step, a 2'-->5' phosphodiester bond is formed between the ____ and the ____. In the second step, a 3'-->5' phosphodiester bond is formed between the ___ and the ___.

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BIO130H1 Lecture 8: Lecture 8 Part 2 (lec 9)

BIO130H1 Full Course Notes

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