CSB349H1 Lecture Notes - Dna Profiling, Transposase, Minisatellite
4 Oct 2012
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Document Summary
99% of the euchromatin and several million bps still not sequenced. Most was repetitive dna very hard to sequence! Most that was sequences was also repetitive elements transposons (~45%) Tandem: the repeats are right beside each other. Interspersed: repeats are not near each other, can be anywhere in the genome. Satellite: huge sequences, centromere (heterochromatic) important for chromosomes! Microsatellite: very small in basepairs and repeats, incredibly variable between individuals, used in dna fingerprinting in crime scenes, etc. Transposons: dna jumping genes, have a transposase gene. Measuring copy number with pcr (for microsatellites, small repeats) Use a primer pair (with known lengths) on either side of the repeated element. Primer is the non-repetitive sequence flanking the repeated microsatellite. Do the pcr and then run a gel. The dna will show up as a bunch of bands but approximately the same sizes.
