CSB202H1 Lecture Notes - Knockout Mouse, Human Serum Albumin, Inflammasome
3 Jun 2013
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Document Summary
Adjuvant perhaps makes the protein last longer in tissue it was unclear what was happening mouse experiment ovalbulmin alone ovalbulmin with adjuvant antibody responses higher with adjuvant. Change ph allow protein to cyrstallize in association with aluminum salt used as vaccination strategy. Human study looking at diphtheria immunization in fluid form or precipitated with alum salt done in children immunized children with fluid or alum-and-diptheria-toxoid. Three injections of fluid 63% of kids had high levels of antibodies; with alum, 94% of kids had antibodies so precipitating antigen with alum salt enhanced immunogenicity. Induces ige skewed t cell response th2 response mainly allergic type of response. Alum induce th1 response also in humans; only th2 in mice. Inducing inflammation one of the typical inflammatory cytokines interleukin1. Il1 is part of inflammasome typical inflammatory induction pathway interleukin one and il18 are both cleaved by caspase one for activation. Aluminum forms crystals first clue to alum.
