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Lecture

CSB327 Lecture 1 Summary

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Department
Cell and Systems Biology
Course Code
CSB327H1
Professor
Maurice Ringuette

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CSB327 Lecture 1 Notes
- ECM
o An ECM is an integrated circuit of complex matrix molecules
o Two major types
Basal lamina or basement membrane
Thin and sheet-like
Underlies epithelium
Surrounds muscle cells, adipose cells and peripheral nerves
Connective tissue ECM or interstitial ECM
Thick
Underlies basal lamina
Most abundant
Composite of all the major types of ECM molecules
o Both are enriched in different types of collagens
- ECM molecules
o Have structural and regulatory activities critical for the development of multicellular organisms
Serve as adhesive scaffold for cell attachment and migration
Act as regulators of all cellular activities
o Dynamic reciprocity is communication between cells and their ECM
o Matrix homeostasis is fundamental to development
The ECM is continuously being remodelled during early development
- Secretory pathway for ECM molecules
o Nucleus pre-MRNA or hnRNA splicing and capping cytosol mRNA translation
translation arrest ER Golgi secretory vesicles secretion extracellular space
- Synthesis of a protein destined for the ER
o Translation begins in the cytosol
RNA is translated in the 5’ to 3’ direction
Nascent polypeptide protein is synthesized from the N-terminus to C-terminus
o When about 70 amino acids have been polymerized, the signal peptide is no longer masked by
the ribosome
Signal peptide
Continuous stretch of 6-30 hydrophobic residues followed by one or more basic
residues in the N-terminus
Begin with a short stretch of positive residues
Direct protein to ER membrane
Open translocon
o SRP (signal recognition particle) binds to the signal peptide on nascent polypeptide
Causes block in translation
SRP is made of six proteins and one RNA
SRP54 has hydrophobic Met residues that binds to the hydrophobic domain of
signal peptide
o SRP54 hydrophobic interaction with signal peptide
o SRP attaches to SRP receptor on RER membrane
SRP and SRP receptor (SRα and SRβ) are displaced and recycled
3 GTP 3 GDP + 3 Pi
o Co-translation translocation

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Description
CSB327 Lecture 1 Notes - ECM o An ECM is an integrated circuit of complex matrix molecules o Two major types  Basal lamina or basement membrane  Thin and sheet-like  Underlies epithelium  Surrounds muscle cells, adipose cells and peripheral nerves  Connective tissue ECM or interstitial ECM  Thick  Underlies basal lamina  Most abundant  Composite of all the major types of ECM molecules o Both are enriched in different types of collagens - ECM molecules o Have structural and regulatory activities critical for the development of multicellular organisms  Serve as adhesive scaffold for cell attachment and migration  Act as regulators of all cellular activities o Dynamic reciprocity is communication between cells and their ECM o Matrix homeostasis is fundamental to development  The ECM is continuously being remodelled during early development - Secretory pathway for ECM molecules o Nucleus  pre-MRNA or hnRNA  splicing and capping  cytosol  mRNA  translation  translation arrest  ER  Golgi  secretory vesicles  secretion  extracellular space - Synthesis of a protein destined for the ER o Translation begins in the cytosol  RNA is translated in the 5’ to 3’ direction  Nascent polypeptide protein is synthesized from the N-terminus to C-terminus o When about 70 amino acids have been polymerized, the signal peptide is no longer masked by the ribosome  Signal peptide  Continuous stretch of 6-30 hydrophobic residues followed by one or more basic residues in the N-terminus  Begin with a short stretch of positive residues  Direct protein to ER membrane  Open translocon o SRP (signal recognition particle) binds to the signal peptide on nascent polypeptide  Causes block in translation  SRP is made of six proteins and one RNA  SRP54 has hydrophobic Met residues that binds to the hydrophobic domain of signal peptide o SRP54 hydrophobic interaction with signal peptide o SRP attaches to SRP receptor on RER membrane  SRP and SRP receptor (SRα and SRβ) are displaced and recycled  3
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