CSB328H1 Lecture 2: Lecture 2 - FROG EGG and FATE MAP
17 Oct 2017
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Egg coated by vitelline membrane and gelatinous coat. Early embryonic cells that undergo cleavage divisions = Used vital dye (nile blue) that does not interfere with embryo development. Prepared agar and stained with the dye. He would cut out pieces and place them on a new agar. Led to a surprising result: areas he stained close together in the early embryo, in the late embryo the stained areas would end up in opposite ends. Cells he had stained on the outside end up on the inside of the embryo. Every time he would get the same result, which means cells develop the same way. Light blue: develops into epidermis skin. Fate map for xenopus blastula ***** know this***** Remains in an inactive state until: activation by uv light, excite by blue light, emission of green light. Zebrafish embryo carries a pa-gfp transgene all cells make a pa-gfp protein, but only cells with activated pa-gfp show green fluorescence.
