CSB331H1 Lecture Notes - Lecture 8: Ptk2, Fakfak, Focal Adhesion
11 May 2015
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Document Summary
Model connecting pi3- k activation and actin. Activation of actin filament assembly by. Regulation of actin nucleation by rac1. Rac1 and cdc42 have emerged as key regulatory players in the formation of these structures. Rac1 can promote actin polymerization at the leading edge of cells by activating wave, an activator of the arp2/3 complex. However, aactivated rac1 does not bind directly to. Wave; instead it binds to sra- 1 which binds to a complex consisting of nck- 1, abi- 1 and wave. Rac1- wave complex then binds to and activates the arp2/3 complex, promoting the formation of an actin branched network. Recently it was elucidated how gtp- loaded, active rac is linked to wave mediated lamellipodia protrusion and membrane ruffling. This entire complex was, by systematic. Rnai- mediated knockdown of each component, shown to be essential for rac- dependent formation of lamellipodia and membrane ruffles. Keratocyte with actin filaments labeled in red by fluorescent phalloidin and the arp.
