CSB351Y1 Lecture Notes - Lecture 34: Nucleoprotein, Paramyxoviridae, Filoviridae
26 May 2018
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Lecture 34: Paramyxovirus II
Nipha and Hendra Virus
• Wide range of hosts and high mortality rates in humans (emerging viral infection)
• Fruit bats natural hosts of both viruses but do not get ill (reservoir)
• 7 viral proteins:
- NP nucleoprotein, L protein viral polymerase, VP30 and VP35 structural proteins (V30 a minor nucleoprotein
intimately associated with RNP, VP35 loosely associated with RNP)
- Glycoprotein GP – mediate viral attachment and entry to cell (n and o-linked oligosaccharide structures)
- VP40 – prominent structural protein, role as matrix protein
- VP24 – membrane protein
- SGP – protect virus from immune system (decoy antigen, interfere with neutrophils, immunosuppressive)
Filovirus – -ssRNA non segmented (large)
• DNA vaccines much more efficient since viral vaccines interfere with immune system
Ebola Virus
• RNA editing used to make two GP from same gene – shorter protein (with no transmembrane domain and is a
secreted SGP) and larger protein (RNA poly stutters to ad extra A and change reading frame)
- Shorter ids atiodies to suppress iue respose ut does’t ativate arophages or edothelial
• Maculopapular (raised spots) rash, thrombocytopenia (lower platelets) and hemorrhagic manifestation, rash
peeled away, lesions/necrosis in almost every organ (liver and spleen most noticeable) and death by shock
• Cytokine storm – body releases toxic cytokine = shock
• Antibody dependent enhancement – C1q of cell surface receptor promote binding of virus to ebola-specific
receptors or endocytosis of target cell
• Natural host (plant) → Original host → animal → human → human
find more resources at oneclass.com
find more resources at oneclass.com
Document Summary
Nipha and hendra virus: wide range of hosts and high mortality rates in humans (emerging viral infection, fruit bats natural hosts of both viruses but do not get ill (reservoir, 7 viral proteins: Np nucleoprotein, l protein viral polymerase, vp30 and vp35 structural proteins (v30 a minor nucleoprotein intimately associated with rnp, vp35 loosely associated with rnp) Glycoprotein gp mediate viral attachment and entry to cell (n and o-linked oligosaccharide structures) Vp40 prominent structural protein, role as matrix protein. Sgp protect virus from immune system (decoy antigen, interfere with neutrophils, immunosuppressive) Filovirus -ssrna non segmented (large: dna vaccines much more efficient since viral vaccines interfere with immune system. Ebola virus: rna editing used to make two gp from same gene shorter protein (with no transmembrane domain and is a secreted sgp) and larger protein (rna poly stutters to ad extra a and change reading frame)
