CSB428H1 Lecture Notes - Lecture 19: Colorectal Cancer, Progenitor Cell, Telomerase

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Published on 15 Aug 2016
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BIOB11 Thursday, July 21
Lecture 19: Biology of Cancer
Slide 2
Lung tissue and colorectal tissue
- both epithelial type tissues
- things that can be controlled in terms of things you breathe and inject
Slide 3
Density-dependent growth
- when flask/culture disk fills up cells will send signals to each other that its getting
crowded, lots of toxic waste being builtsignal to stop cell cycle so less of them and less
toxic waste being let out
Density-independent
- just keep going
Contact inhibition
- when touch each otheragain signal to stop dividing
- cancer cells will start to pile up on each other
Anchorage
- contact with membrane or other cells
Slide 5
Fig 16-4
- w/o growth factors normal cells won't get signal to divide (dotted blue line)
- if you add GFs back in see solid blue linebeing to grow again
Slide 6
Normally if something wrong happenssignal to apoptosis
Telomerase important for integrity
- cancer cells have telomeric activity reactivated
Gap jxns
- cancer cells do not really communicate w/ one another
Slide 7
Proteinaseenzyme that destroys proteins
- can break down ECM
Metastasize
- if tumour is secreting more proteinases than normalbreaks ECMtumour pieces can
break off go into places like lymphatic system/circulatoryset up secondary colonies
- 'spreading of cancer'
Slide 8
Benign tumour--not cancerous
find more resources at oneclass.com
find more resources at oneclass.com
BIOB11 Thursday, July 21
Lecture 19: Biology of Cancer
- cells gotten out of control in some way (unresponsive to growth controls)
- lack ability to invade other tissues
Slide 9
Stem cells very powerful//divide slowly//self-perpetuate//can divide into pluripotent
progenitor cell
- differentiate into any cell type
Stem cells good place for cancer cells to arise
If cancer doesn't arise from stem cellneed to acquire ability themselves (one mech is the
telomerases)
Slide10
Oncogenic viruses
- oncogenes associated w/ normal cell cycling--but extra copy of gene could lead to down
path that oncogenesis might occur
Slide 11
Popl'n level
Slide 12
Cellular level
Fig 16.20
- progressive type of thing (muts accumulate)
Slide 16
Exposure to coal tar showed nothing in terms of tumors
Slide 17
Tried exposing to croton oil
- exposure multiple times over course of 30w period
None dvlp'd tumors
Slide 18
Exposure to both
In 30weeks 100% all had tumors
Slide 19
Each chromosome is displayed in there showing its genes
Both show two diff colorectal cancer patientsdiff sets of muts
'drivers'know in commonly effected cancer causing genes
find more resources at oneclass.com
find more resources at oneclass.com
BIOB11 Thursday, July 21
Lecture 19: Biology of Cancer
- causal relationship
'passenger'over course of time accumulated muts
Slide 20
Microarrays to look at gene expression in cancer cells
- maybe inform us of how to treat patients
Slide 22
Each column is invid patient
- going down dif genes
Both types of patients have diff mut profile that leads to similar cancer
Slide 23
Collective knowledge of expression profile that will identify an indiv as a patient w/ good
or bad signature/outcome from cancer
Person can be lymph node (-) and depending on type of cancer the gene expression
profile can put them in poor signature
- would want a more aggressive tx
Want treatments using microarrays could lead to (making a decision to treat a (-) patient
with something more)
Slide 24
Positive patients may get more aggressive tx than they actually need
find more resources at oneclass.com
find more resources at oneclass.com

Document Summary

Things that can be controlled in terms of things you breathe and inject. When flask/culture disk fills up cells will send signals to each other that its getting crowded, lots of toxic waste being built signal to stop cell cycle so less of them and less toxic waste being let out. When touch each other again signal to stop dividing. Cancer cells will start to pile up on each other. W/o growth factors normal cells won"t get signal to divide (dotted blue line) If you add gfs back in see solid blue line being to grow again. Cancer cells do not really communicate w/ one another. If tumour is secreting more proteinases than normal breaks ecm tumour pieces can break off go into places like lymphatic system/circulatory set up secondary colonies. Cells gotten out of control in some way (unresponsive to growth controls) Stem cells very powerful//divide slowly//self-perpetuate//can divide into pluripotent progenitor cell.