IMM250H1 Lecture Notes - Lecture 7: Reverse Transcriptase, Gp41, Envelope Glycoprotein Gp120

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Published on 14 Apr 2013
School
UTSG
Department
Immunology
Course
IMM250H1
IMM250 Lecture 7 HIV
Slide 5 So what is a retrovirus?
HIV = retrovirus = RNA virus = RNA is infectious
Make vaccine that interfere with viral life cycle but not host (RNA DNA does not happen in
host cells)
Slide 8 Animal origins of HIV
Highly related virus with the same symptoms but present in different species
Requires blood contact for transmission
No HIV epidemic because transmission of HIV is limited
We must continue to vaccinate the human population
Slide 9 The HIV virus
Two copies of RNA
Reverse transcriptase (RNA as template DNA)
gp41 and gp120 are encoded by HIV
Slide 10 The retroviral life cycle
30% of genome is made of provirus (double stranded DNA integrated into host chromosome)
May or may not elicit symptoms
Slide 11 HIV infection of target cells
gp120 (HIV) binds to CD4 (T cells)
gp120 conformational change
gp120 (HIV) binds to CCR5 or CXCR4 (T cells)
gp41 conformational change
gp41 (HIV) binds and fuses with membrane
RNA is delivered into the cell
Slide 13 Reverse transcription of viral RNA (RNA DNA)
DNA-RNA hybrid is unstable
Host enzymes degrade RNA
Second strand synthesized by DNA polymerase
Reverse transcriptase is one of the original targets for retrovirus
Slide 15 Proviral DNA is transcribe to generate viral RNA and proteins
LTR required for reverse transcriptase
5’ LTR contains strong promoter sequences that drive the transcription of genes
Slide 16 Budding of new virus from infected cells
gp160 is cleaved by a protease to create gp120 and gp41
Slide 18 HIV can cause lysis of CD4 T cells
Decline in 50% of CD4 T cells is still functional
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Document Summary

Hiv = retrovirus = rna virus = rna is infectious. Make vaccine that interfere with viral life cycle but not host (rna dna does not happen in host cells) Highly related virus with the same symptoms but present in different species. No hiv epidemic because transmission of hiv is limited. We must continue to vaccinate the human population. Reverse transcriptase (rna as template dna) Gp41 and gp120 are encoded by hiv. 30% of genome is made of provirus (double stranded dna integrated into host chromosome) Slide 11 hiv infection of target cells. Gp120 (hiv) binds to cd4 (t cells) Gp120 (hiv) binds to ccr5 or cxcr4 (t cells) Gp41 (hiv) binds and fuses with membrane. Slide 13 reverse transcription of viral rna (rna dna) Reverse transcriptase is one of the original targets for retrovirus. Slide 15 proviral dna is transcribe to generate viral rna and proteins. 5" ltr contains strong promoter sequences that drive the transcription of genes.

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