MGY277H1 Lecture Notes - Lecture 2: Glycan, Flagellin, Enzyme
2 Apr 2017
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Easy to observe cell size, shape and motility (alive) 1000x more resolution reveal fine details of cell structure. Limit must be in vacuum (air molecule would interfere with e-), b&w image, cell must be dead, toxic stain. Use lenses to bend light for focusing and enlarging a small object. Light is either directed onto or passed through the sample been viewed. Physical phenomenon when light rays change direction due to change in the medium through which they travel. Lenses use refraction to focus light, and light bend only at the boundry of the medium. Refraction index measure of relative speed of light as it passes through a medium. Refraction is the bases for light microscope, the bend is sharper when the light hits at an angle. 3 basic parameter of all microscope: mrc. Magnification the increase in the apparent size of the object compare to the size of the actual object.
