MGY378H1 Lecture Notes - Helper Virus, Deoxyadenosine, Transgene
3 Jun 2013
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Document Summary
Intracellular parasites that have evolved to efficiently transfer their host genes into cells. Retroviruses most predominant can integrate their genes into the host genome get stable gene expression can also get high gene expression can make pseudotype viruses express and use correct promoter . Retroviruses long term stable expression integrate their genes into host genome. Expression of short hairpin rna use retrovirus to express this which will knock out expression of specific gene. Gene therapy is continuing introduce healthy gene in cells with defective gene, or for cancer treatment (introduce toxic gene to kill cancer cells); can tag a cell to see how things developed . Vaccines virus vectors as vaccines b/c can put in gene of interest and induce immune response in host. High production of virus vector target cell, 10^13 high levels of virus. Delivery majority of viruses do not target specific cells want to hit only desired cells.
