2 Pages
Unlock Document

University of Toronto St. George
Molecular Genetics and Microbiology
Timothy Hughes

2012.10.04 ENCODE  Most of the functional DNA appear to be regulatory elements  Top down science  K562 a common cell line used in research  RNA-seq, ET, CAGE are the most commonly used for transcript identification  DNAse-seq is the big one for chromatin accessibility identification PET - Used for genome sequencing - Long pieces of DNA and just sequence the ends of them can then assume the middle part of the ends is transcribed - Also can know the size of the transcript - Take fragements and clone them into vectors have restriction sites (type 2, don’t cut at the site, it reaches over the site and cut) - Yellow parts are constant CAGE= Cap Analysis of Gene Expression - ? - RNA-seq is more accurate for expression level: the above techniques are useful for identifying the location of sequence but not ID the sequence DNAase-seq - MMase 1 cut nucleosomal DNA - DNAses 1 can cut nucleosomal DNA but can’t get into chromatin - DNAse 1 accessible= open chromatin - DHS (DNAse hypersensitive site) - Whole genome is coated with nucleosomes - Not against the rules to have nucleosomes in the empty space becuz DNAse can cut nucleosomal DNA - MMase1 often picks up flanking region of the empty space: Nucleosomes carrying specific modification of regulation site distinct nucleosome peak at assay and this is where MMase cuts Paper - Integration: compare tracks to each other
More Less

Related notes for MGY299Y1

Log In


Don't have an account?

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.