MGY299Y1 Lecture : Notes taken during lecture

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redtermite204

19 Dec 2011

School

UTSG

Department

Molecular Genetics and Microbiology

Course

MGY299Y1

Professor

Johanna Rommens

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Document Summary

Can pass from one species to another; from one bacterium to another. Broad host ranges self-conjugative plasmids have. Needs conjugative plasmid that codes for pillus: ex. F factor: finds receptor on f-, draws the two bacteria together so cell walls touch then genetic material passes through. In our hfr experiment, screen for five different factors. Mechanism: single stranded dna goes into recipient. complementary strand made in both donor and recipient. Requires tra genes, encoded on conjugative plasmid. Large plasmid f: single copy, tra last genes to be transferred. Important in hfr transfer because entire bacteria chromosome come between the tra genes and the ones that are transferred before tra genes y. In these cases, tra genes not likely to be transferred: room on plasmid and in the genetic mechanism of transfer for other genes to be carried. same is element present on conjugative plasmid can also be present on chromosome of the recipient bacterium y.

Related Questions

A conjugation experiment is carried out bewteen two bacteria.The original recipient is antibiotic resistant, but can not grow inthe absence of Thr, Phe and Leu. After 5 minutes of conjugation,the cells can grow without Phe or Leu. After 10 minutes ofconjugation, the cells can grow on plates without Thr, Leu or Phe.What is the correct order of these genes?

A. Thr, Phe, Leu

B. Phe, Leu, Thr

C. Leu, Phe, Thr

D. can not tell from this info

fuchsiagrasshopper6

Select all of the TRUE statements. (Select all that apply.)

		The direct movement of DNA between two bacteria of the samegeneration is called horizontal genetic transfer.
		In conjugation, the strand of DNA that is transferred from thedonor cell to the recipient cell is single stranded.
		The process of conjugation may introduce new genes into therecipient strain, altering its genotype.
		A cell with an F factor integrated into the bacterial chromosomeis called F+.
		An episome is a segment of bacterial DNA that can exist as aplasmid or be integrated into the bacterial chromosome.
		During transformation, homologous recombination may occur if thesequence of the introduced DNA has no similarity to the hostDNA.

yellowgoat340

A mutation in the gene encoding the enzyme that cuts F factor DNA during conjugation would result in:

a. an inability to separate the recipient DNA from the donor DNA
b. an inability to draw the conjugated cells close enough to allow DNA transfer
c. the transfer of single-stranded DNA instead of double-stranded DNA
d. the inability of the donor DNA to move through the pilus
e. the inability of the recipient cell to replicate the F factor after transfer

MGY299Y1 Lecture : Notes taken during lecture

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