Theres also the secretion signal that tells it where to go. The bacteria use these proteins to manipulate the actin cytosksleton either enhancing or deteririoating, and these type 3 effectors can block digestion of bacteria by blocking the action of the digestive enzymes. At the base, theres an atpase that pumps them out. The chaperone bound to an effector will bind to the atpase, the chaperone will leave and the atpase will pump out the protein. It picked up 2 big gene blocks via horizontal gene transfer that can make the 2 type 3 secretion systems that are used to attack eukaryotic cells. Salmonella, unlike bacteria in a soil or water, when it touches a eukaryotic cell, if its spi1, it can inject proteins into the clel which make the cell engulf the salmonella. It uses its spi-1 to get itself inside the clel, and it then uses spi-2 to get inside a internal vescicle.