Notes taken during lecture

5 Pages
95 Views

Department
Molecular Genetics and Microbiology
Course Code
MGY378H1
Professor
Alan Cochrane

This preview shows pages 1 and half of page 2. Sign up to view the full 5 pages of the document.
Description
Jan 27 2012reoviridae Lot of dsRNA genomesreoviridiaevirsues isolated in 1950s from kids andInfect range of specieshumans insects plants yeastquite diverse in what they challenge Reoviridaetop three what will be discussedOrthorevovirusesrespiratory entericcan isolate from either respiratory tract or gutCalled orphan viruses bc in humans no pathologydefinitely not lethal There serotypesperhaps fourth And mild diarrheaRotavirusescan cause illness in humans Coltiviruseshave RARE cases where caused disease in humans Reovirus properties No envelope Entirely protein and nucleic acid Actually two concentric shellsouter and innerprovides stability in env that not normally seen Shellstreat virion with partial proteolysisget intermediate subviral particle chew back more get viral core This virus seen in wildisolated from multiple sourceso Ingested contaminated foods soil etcinfected this wayo Virionuninfective fairlyo Nust be processed into intermediate to be infectiouso But if proteolysed into core becomes non infectious again Stripping off layers of protein is whaqt proteolysis is doingo One of the first to come off is sigma 3 that forms outer shell leaving behind sigma 1 and mu 1o Mu 1 and sigma 1 losscore results Propertieso Genomes dsRNAo Also segmentedten to twelve segmentso Range in sizegenome segments are in generaldo not have caps on them but produce mRNA that do in some in stanceso Break down into sizeo L M and S Three of L coding for lambda proteins Three of M that code for mu proteins Smallfour ofcode for four sigma proteins Functions Genotype reovirus based on nucleic acido Three serotypes of virus in gel electrophoresiso Run on gelby differences in sizing of different segments can genotype this Virus lives in environmenttaken up by ingestion of contaminated water food
More Less
Unlock Document

Only pages 1 and half of page 2 are available for preview. Some parts have been intentionally blurred.

Unlock Document
You're Reading a Preview

Unlock to view full version

Unlock Document

Log In


OR

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


OR

By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.


Submit