MGY440H1 Lecture Notes - Interleukin 8, Tight Junction, Confluence
3 Jun 2013
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Document Summary
Adenovirus receptor interaction can be anywhere on cell - top surface, bottom surface, etc. Done in studies with cell culture - usually without polarized cells. Polarized cells = distinct sides (have apical surface, basal-lateral side) Confluent cells in culture - may still have tiny spaces even though appear confluent whereas in reality, intact epithelial layer, with zero spaces (tight junctions, etc. ) When cell is polarized, no integrins, no car on surfce - integrins on bottom, Car part of tight junction (between cells, adhesion molecule helping hold cells together) How does the infection establish itself? constantly used. Abrasions can occur on for instance hands, because they are. Macrophages (alveolar macrophages) take up viruses etc. Two differen cell types - open bars are a549 cells, black bars are. Edta = chelates calcium, which is necessary for adhesion. Cannot assess infection by cpe because cannot see the cells once. Polarized cells - adding virus from apical surface - hardly any infection.
