PCL102H1 Lecture Notes - Lecture 8: Big Data, Data Analysis, Base Pair

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The genome of 3x10^9 bp into chunks of 300bp. The sequences are now knows because there are adaptors on them now. On this slide there are dna primers on it. The adaptor sequences can stick onto the primers due to complimentarity. We need to start with single stranded dna so the primers can bind. Its totally random where these pieces of dna end up. We add primers and more nucleotides to make the dna more visible, we make them into groups. Doing this makes lots of copies of the single piece of dna. The specialized nucleotides make them able to stop the chain, but they can also start the chain again. This is why the illumine method is so special. Each dot of colour represents a cluster of dna templates. We keep synthesizing all the way down until we get to the bottom, and on each base pair a colour will flash.

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