PCL486H1 Lecture Notes - Lecture 2: Apoptosis, Metaphase, Spindle Apparatus
26 Feb 2017
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These molecules will bind to kit ligand or stem cell factor. When a process is turned on and this ligand is secreted dimerization of two of these kit molecules: then a conformational change. Dimerization of the ligand and recruitment of the two kit rtk receptors: followed by a conformational change which allows for their trans-auto-phosphorylation, kinase domains come close together. Akt substrates: all roads lead to proliferation and survival. Ubiquitylation process modulated by a protein called slc1. The scf (skp2, cullin, f-box) complex is a tetrameric e3 ligase. Phosphorylated substrates are recognized by the cd4 or skp2 subunits. Skp2 (cdc4) binds to slc1 only when it is "polyphosphorylated": monophosphorylation = no interaction. Interaction between wd40 and the phosphorylated slc1 allows e3 ubiquitinylation. Takes a lot of energy to make a protein: full commitment by the kinases (phosphorylation) before we go ahead with the degradation. Slc1 inhibits entry into the cell cycle.
