PSL450H1 Lecture : Sept 25 lec

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13 Apr 2011
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The acceptor can glycosylate but doesn"t have proteins: g protein is sensitive to endo h. golgi extracts. Longer u leave the wt protein, the more resistant the protein is: pg 4 slides 1: If trated wid poison it kills the activity. So he takes poisoned cytosol and adds fractions ofnon poisoned cytosol. One protein, called nsfprotects the cytosol from: one protein it had all the activity in it. So the actual protein is nsf in active form. First component of fusion machinery: complementary approaches: Eg. fusion machinery must include sth on synaptic vesicles. Mutation in yeast that didn"t secrete properly: nsfstructure: N terminis domain is protein protein interaction: nsf binds to membranes vis asnap. Atp ase can only bind to membranes in presence of non hydrolysable atp and sth else. Sth on the membranes that nsf and snap stick to. Snap receptor so he called them the snares. now he wanted to find snares. (diagram)

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