RLG100Y1 Lecture : Lecture on Judaism-October 2

Chapter 10

1.Outline the history of our knowledge on DNA up to Watson and Crick. What were the main contributions made by each researcher’s key experiment?

2.Explain the setup of the Hershey and Chase experiment, what would the results have been if protein was the genetic material?

3.Draw the structure of a DNA nucleotide, labeling each main component correctly. How does an RNA nucleotide differ?

4.If a section of double stranded DNA contains 19% Adenine, how much Thymine is present?

5.You are a researcher studying the genetic basis of heart attacks and have been working to determine the expression levels of different genes that might contribute to cancer formation. You obtain the DNA methylation status of five genes of interest (the data are shown in the table below). The plus (+) sign indicates the level of DNA methylation; more plus signs correlates with increased methylation levels.Based on this information which genes would you predict to have the highest rate of transcription?

What are the characteristics of the 3 main DNA forms?

Chapter 11

What are the different types of chromatin?

What are the structures and important roles for telomeres and centromeres?

What are the differences found between eukaryotic chromosomes and mitochondrial?

Chapter 12

Explain each of the different models of replication.

If you grow a culture of bacteria in media with radioactive nucleotides so that all DNA in the cells include radioactive nucleotides and then place the bacteria in new non radioactive media. After two rounds of replication what proportion of the DNA molecules will contain radioactivity?

Summarize the similarities and differences between rolling-circle replication, theta replication and linear eukaryotic replication.

What are the functions of the different DNA polymerases found in eukaryotic cells?

Draw a replication fork and include all key components and orientations. (Leading/lagging strands, DNA helicase, RNA primer and DNA gyrase)

What is the Holliday model of recombination and what are the necessary steps?

Chapter 13

What are the different types of RNA and what roles do they play?

Describe the properties and functions of each of the RNA polymerases and how they differ depending on the organism.

Describe in detail the process and mechanisms of transcription in both prokaryotes and eukaryotes.

Chapter 14

What are the primary purposes of each of the three post transcriptional modifications that occur in eukaryotic cells.

What is alternative splicing and what role does it play in the cell?

How is ribosomal RNA processed after transcription?

How do siRNA and miRNA work, describe/draw out the process in detail.

can you please give me a response for this Article(type the anwer)

When a soil dries out, this has a negative impact on the activity of soil bacteria. Using an innovative combination of state-of-the-art analysis and imaging techniques, researchers at UFZ have now discovered that fungi increase the activity of bacteria in dry and nutrient-poor habitats by supplying them with water and nutrients. The ability of fungi to regulate drought stress in soil and thus sustain ecosystem functions is an important insight in the context of climate change.

Many fungi spread through the soil with a fine network of thin filaments known as hyphae. In their search for water and nutrients the hyphae grow in different directions, constantly enlarging the network. Once found, water and nutrients are absorbed and transported through the hyphae, allowing them to be supplied to parts of the fungal network in dry or nutrient-poor areas of the soil. But it isn't only the fungus itself which benefits from the transport of material through the hyphal pipelines: bacteria, too, are kept supplied with the water and nutrients they need to thrive. This has now been demonstrated by a team of UFZ researchers in a study recently published in the journal Nature Communications. "We've suspected for a long time that fungi play an important role in the soil moisture budget," says UFZ environmental biotechnologist Prof. Matthias Kästner. "Now, using secondary ion mass spectrometry techniques (NanoSIMS and ToF-SIMS) from the ProVIS research platform at UFZ, we've finally obtained experimental evidence."

As part of their investigations, the researchers closely examined the transport of water, substrates and nutrients through the microscopically small hyphae of fungi. They grew the fungi on a culture medium of water, glucose and nitrogen-containing nutrients. The fungal hyphae had to pass through a dry, nutrient-free zone in order to grow through into a new area containing the culture medium. The inhospitable transition zone contained spores of the common soil bacterium Bacillus subtilis. Spores are inactive stages of Bacillus that form when there is insufficient water, food and nutrients available for bacterial growth. The bacteria go into a kind of dormant stage, from which they only awake once the environmental conditions are more favourable for living again.

In the experiment, these conditions were indeed improved by the growth of the fungi: "As the fungal hyphae grew through the dry zone, the bacterial spores germinated and we noticed clear microbial activity," says UFZ environmental microbiologist Dr. Lukas Y. Wick. "The fungi obviously improved the environmental conditions for the bacteria and woke them from their slumber, like Sleeping Beauty." But what exactly happens at a chemical level when fungal hyphae grow in direct proximity to bacterial spores? To answer this question, the researchers 'labelled' the water, glucose and nitrogen-containing nutrients in the culture medium in advance with stable isotopes. If these substances were transferred from the fungus to the bacteria, they could be detected using the isotopic marker and the NanoSIMS method, with nano-scale spatial resolution. "The NanoSIMS method reveals the distribution of elements and isotops and therefore allows us to observe metabolic processes," Kästner explains. "As it turns out, we did find the stable isotopes of the labelled water, glucose and nitrogen-containing nutrients in the cell mass of the bacteria -- which could only have come from the fungi."

This study has given the UFZ researchers another important insight into fungi and their important function in soils. Fungi serve as pumping stations and pipelines for water, substrates and nutrients and can colonise inhospitable locations, making them habitable for bacteria -- and thus stimulate microbial activity in the soil. In previous work the researchers have already shown that fungal hyphae act as a kind of fungal highway for bacteria, allowing them to move around, and provide a hotspot for bacterial gene transfer. Wick continues: "The results of this latest study demonstrate once again that, through their interaction with bacteria, fungi play a significant, previously underestimated role in the soil ecosystem."

If a soil is contaminated with pollutants, for example, bacteria can break them down. But if the soil is too dry, the degradation processes will come to a standstill. "If the drought period lasts for a limited time, fungi have a stabilising effect and can keep soil processes going. This could be important specifically with regard to the impacts of climate change, if the ratio of dry to moist areas of soil dramatically increases," says Kästner. In future investigations, the researchers therefore intend to look even more closely at soil as a true ecosystem. "We want to carry out soil experiments under different environmental conditions and find out what influence fungal growth has on the breakdown of pollutants," says Wick. He adds: "It's important to have a better understanding of the role of fungi in the soil ecosystem. Only when we know how soil works can we respond to changes, for example those caused by climate change, with informed decisions."

Chapter 2 Conceptual Framework for Financial Reporting

In other words the objective of financial reporting is_________________________________________________________________________________________________________________________________________.

LO 2.3 Identify the qualitative characteristics of accountinginformation. The qualitative characteristics of accountinginformation distinguish better and more useful information frominferior and less useful information.

a.Fundamental qualities of useful accounting information.

Relevance._______________________________________________________________________________________________________________________________________________________________

(a)Predictive value.:_____________________________________________________________________________________.

(b)Confirmatory value:_________________________________________________________________________________.

(c)Materiality.:_________________________________________________________________________________.

FaithfulRepresentation.___________________________________________________________________________________________________________________________________________________

(a)Completeness_________________________________________________________

(b)Neutrality_______________________________________________________

(c)Free from error___________________________________________________

b.Enhancing Qualities Enhancing qualities complement thefundamental qualities and include:

Comparability__________________________________________________________________.

Consistency_____________________________________________________________________________________________________________________ Verifiability__________________________________________________________________.

Timeliness__________________________________________________________________.Understandabili_____________________________________________________________________________________________________________________

Select the qualitative characteristics for the followingstatements.

(a) Quality of information that assures users that informationrepresents the economic phenomena that it purports torepresent.

(b) Information about an economic phenomenon that corrects pastor present expectations based on previous evaluations.

(c) The extent to which information is accurate in representingthe economic substance of a transaction.

(d) Includes all the information that is necessary for afaithful representation of the economic phenomena that it purportsto represent.

(e) Quality of information that allows users to comprehend itsmeaning.

SFAC No. 8 identifies the qualitative characteristics that makeaccounting information useful. Presented below are a number ofquestions related to these qualitative characteristics andunderlying constraint.

(a) What is the quality of information that enables users toconfirm or correct prior expectations?

(b) Identify the pervasive constraint developed in theconceptual framework.

(c) The chairman of the SEC at one time noted, “If it becomesaccepted or expected that accounting principles are determined ormodified in order to secure purposes other than economicmeasurement, we assume a grave risk that confidence in thecredibility of our financial information system will beundermined.” Which qualitative characteristic of accountinginformation should ensure that such a situation will not occur? (Donot use faithful representation.)

(d) Muruyama Corp. switches from FIFO to averagecost to FIFOover a 2-year period. Which qualitative characteristic ofaccounting information is not followed?

(e) Assume that the profession permits the savings and loanindustry to defer losses on investments it sells because immediaterecognition of the loss may have adverse economic consequences onthe industry. Which qualitative characteristic of accountinginformation is not followed? (Do not use relevance or faithfulrepresentation.)

(f) What are the two fundamental qualities that make accountinginformation useful for decisionmaking?

(g) Watteau Inc. does not issue its first-quarter report untilafter the second quarter’s results are reported. Which qualitativecharacteristic of accounting is not followed? (Do not userelevance.)

(h) Predictive value is an ingredient of which of the twofundamental qualities that make accounting information useful fordecision-making purposes?

(i) Duggan, Inc. is the only company in its industry todepreciate its plant assets on a straight-line basis. Whichqualitative characteristic of accounting information may not befollowed?

(j) Roddick Company has attempted to determine the replacementcost of its inventory. Three different appraisers arrive atsubstantially different amounts for this value. The president,nevertheless, decides to report the middle value for externalreporting purposes. Which qualitative characteristic of informationis lacking in these data? (Do not use relevance or faithfulrepresentation.)