MICR 302 Lecture Notes - Lecture 2: Fus3, Ascus, Meiosis
Document Summary
How to measure the output of pheromone detection: changing gene expression. Mating genes in the o state are controlled by dig1 and dig2 proteins that bind to the ste12 proteins. Ste12 = key transcription factors for mating: Cdk inhibitors (far1) = stops cells in g1 (only cells in g1 will proceed to mate arresting cells at this phase is important so that you don"t undergo mitosis and have more than one genome) Ste12 is already bound to the dna, so induction of genes happens quickly after the dig1/2 inhibitors fall o . Fus3 (phosphorylated via the mapk pathway and di usible) will activate the ste12 transcription factors and will induce gene transcription. The rst gene/protein activated is msg5 = a protein phosphatase that acts like brakes for the kinase phosphorylating fus3, turning the mapk signal o by dephosphorylating. Limits the pool of transcribed gene product.