MICR 302 Lecture Notes - Lecture 3: His3, Metabolic Pathway, Selectable Marker
7 Apr 2018
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Document Summary
Gain and loss of function experiments are the major driver in genetic analysis: Loss of function (lof) = remove a part and see what it causes, gain of function (gof) = add more parts to gain more function. Yfg1 = complete deletion/knockout leading to a loss of function. Yfg1::nat = replaced gene with something to select for (ie. nat = resistance gene) Pgal::yfg1 = allows you to crank up the expression of yfg1 by replacing the normal promoter with pgal which can be controlled by galatose. Yfg1 = protein product of a gene. Gain of function experiments: typically overexpression or inducible overexpression. Auxotrophic markers: lab strains that have a collection of metabolic genes that are disrupted require the disrupted amino acid for growth. Knocking these out allow for the use of plasmid dnas with these markers that will restore the metabolic pathway if inserted.
