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Class Notes for Microbiology at University of Victoria

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MICR 302 Lecture 16: MICR 302 Lecture 16
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Lecture 16 PART 2 SYSTEMS BIOLOGY AND NETWORKS Systems Biology: The study of an organism as an integrated and interacting network of genes, proteins, molecules, and biochemical reactions requires systematic data collect...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 17: Canavanine, Selectable Marker, His3
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Lecture 17 Genetic Interactions - occurs when the phenotype of a double mutant (gene A gene B) is greater that that of single mutants (gene A OR gene B) Fitness ratio: growth rate of mutant/growth rate of wild-type ...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 18: Selectable Marker, Telomere, Epitope
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Lecture 18 SYNTHETIC BIOLOGY THE NEXT FRONTIER: making yeast genomes How to Build a Genome: IDEA: by inserting a knockout in place of normal coding sequence and epitope tag to follow it then undergo homology recombination ...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 15: G1 Phase, Mutagen, S Phase
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Lecture 15 Classic Yeast Genetic Screens pathways failure is the source of most disease, our goal is to understand pathways and their regulations to understand why they cause mutated phenotypes. THE CELL CYCLE: dened via...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 14: Pheromone, Mating Of Yeast, Fus3
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Lecture 14 S. cerevisiae lifecylcle: 1. a mating event happens between two mating types: Mata and Matalpha (almost genetically identical, physiologically are identical) 2. they mate through conjugation cell wall and PM fu...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 13: Corn Smut, Cryptococcosis, Immunodeficiency
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Lecture 13 Yeasts can be: 1. Symbiotic: - mutually benecial 2. Pathogenic 3. Saprophagic - feed off decaying matter from other plants or organisms NOTE: highly related organisms (what u learn in yeast in beer and bread...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 6: Protein A, Immunoglobulin G, Zygosity
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Protein complexes or the Interactome TAP tags for purication of protein complexes help to identify proteinprotein interactions: Use sequential anity purication rather than singular anity purication: 1. Load the puried p...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 4: Phenotypic Screening, Galactose, His3
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Genetically engineered yeast strains for industry: EXAMPLE: insert 21 genes into a yeast strain, knockout 3 = generate strictosidine in yeast (normally derived from plants) that is normally expensive. Counterselectable ...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 5: Reverse Transcriptase, Ribonucleotide Reductase, Northern Blot
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Traditional Molecular Biology vs. Systems biology Traditional molecular biology: analysis of individual genes, proteins, molecules by studying their specic functions Systems biology: the study of an organism as an integr...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 3: His3, Metabolic Pathway, Selectable Marker
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Gain and loss of function experiments are the major driver in genetic analysis: Loss of function (LOF) = remove a part and see what it causes, Gain of function (GOF) = add more parts to gain more function Nomenclature: ...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 7: Ploidy, Cell Culture, Epitope
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Haploid KO proling: Classical genetics: Researchers generate mutations to understand gene functions disrupt gene networks. Chemical genetics: Researchers use small molecules to understand gene function and perturb ge...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 8: Protein Structure, Genome Evolution, Transfer Rna
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New Frontier: Synthetic Biology building organisms with desirable properties How to build a genome: E.coli 1. Start with short oligonucleotides that are designed with regions of homology that will be synthesized into the...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 2: Fus3, Ascus, Meiosis
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Slides 34 43 How to measure the output of pheromone detection: Changing Gene Expression Mating genes in the o state are controlled by Dig1 and Dig2 proteins that bind to the Ste12 proteins. Ste12 = key transcription fa...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 1: Corn Smut, Saccharomyces Cerevisiae, Yeast
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Micr 302 Budding yeast, a model eukaryote (Chris Nelson) Introduction Yeast is used in the lab, academics, and biopharm companies to try and understand how to develop drugs and processes that relate to disease Fruit will...

Microbiology
MICR 302
Briant
MICR 302 Lecture Notes - Lecture 5: Asymmetric Cell Division, Spore, Sigma Factor
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Topic 5e: Sporulation (Bacillus) - Asymmetrical cell division: - Caulobacter crescents will undergo division into swarmer cells (motlile) and stalked cells (that remain adhesive) > this allows cells to explore ...

Microbiology
MICR 302
Briant

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