BIOL 2131 Lecture Notes - Lecture 8: Nanopore Sequencing, Frederick Sanger, Microscope Slide
14 Mar 2016
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Key questions for week #5 lectures: chapter 3. Understand the differences between the various types of dna sequencing. How microarrays work and uses in diagnostic testing. How hybridization is used in molecular biology techniques: all single stranded nucleic acids can hybridize to one another if they are complementary. How are eukaryotic genes structured and what is the role for introns and exons in gene evolution: prokaryotic genes are not interrupted. 1: just starting to become commercially available, specialized slide with pores and electrical detection, have had some success getting it commercialized, no chemistry, all physics, primarily by a single company called nanopore sequencing. In some cases, you want the entire sequence, in others cases you want selected sequences. Don"t use this to do entire genomes anymore: too long, too expensive. Use four different termination nucleotides: dideoxynucleotides, with no hydroxyl, dda, ddt, ddc, ddg. Mixed in a reaction, all in the same tube, with regular deoxynucleotides that have an.
