BIOC 2010 Lecture Notes - Lecture 8: Disulfide, Cuvette, Cystine
15 Mar 2016
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Memorize which side chains are ionizable and know what they look like when they are protonated and deprotonated. At neutral ph, cys and tyr tend not to be ionized: can be ionized under specific circumstances. Don"t need to know the pka numbers. If you have two sh that are near each other, they can be oxidized to form a disulfide bond (which is a covalent bond) They spontaneously form within proteins: they help to keep proteins together. Reduced form is with the free sulfhydryl sh. Oxidized is the disulfide bond: the two together are called cystine. Amino acids with aromatic side chains have the special property of absorbing uv light. Tryptophan is by far the best absorber of uv light. Ability to absorb uv light is very useful when it comes to analyzing proteins. Absorbance of a molecule as a function of its concentration. The higher the concentration, the more the absorbace will be.
