BIOC 2010 Lecture Notes - Lecture 5: Titration Curve, Grapheme, Titration
15 Mar 2016
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Document Summary
Look at a crystal containing many protein molecules. Neither of the cases were they looking at the individual protein molecule. Molecules change shape and conformation quite dramatically. When you look at a bunch of moelcules, you can"t really see the changes that occur under different conditions. The first thing they had to do was find something to rest the protein on to take an image on it. If it was on glass, they would have to blast it with a relaly high energy beam of electrons: low energy electrons would not penetrate htrough the glass. If you blast the molecule, they would destroy it. They used grapheme: a forme of carbon, a single molecule thick layer of carbon in a hexagonally pattern, very strong, one molecule of carbon thick. Spray a fine mist of protein solution on to the grapheme. Want the proteins to nicely come to rest on grapheme surface with unaltered conformations.
