Anatomy and Cell Biology 4429A Lecture Notes - Lecture 12: Immunofluorescence, Hyperoxaluria, Scurvy
12 Jan 2018
School
Department
Professor
Document Summary
Anfinsen"s: urea and (cid:533)-me denaturation of ribonuclease a and lysozyme, followed by renaturation by removing denaturing-agents. Rudolf shoenheimer: 15n labelled tyr concept of turnover rates and protein lifespans. Dorothy andersen: first defined the cf disease; initially thought to be pancreatic. Dr. tsui: found the cftr gene (1985) using rflp and vntr: rflp: restriction fragment length polymorphism, vntr: variable number of tandem repeats. Deduve + theorell: discovered secretory pathway (er & golgi oxidation compartments: de duve plots: enzyme activity vs. amount of enzyme present in a compartment. Albert claude: developed cell fractionation using centrifugation: nuclei | mitochondria + lysosomes + peroxisomes | microsomes (vesicles) Protein precipitation is more effective in colder temperatures than at room temperatures, because enthalpically driven hydrophobic interactions cause them to aggregate to each other and precipitate. Water molecules are less mobile at lower temperatures and are more likely to form hydrogen bonds with each other around the protein.
