Anatomy and Cell Biology 4429A Lecture : Protein Folding II
22 Dec 2011
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If there is no hsp70 in the cytoplasm, the proteins will not be imported into the mitochondria. Mitochondrial hsp70 pulls the protein across while cytosolic hsp70 starts to release the precursor protein. *hsp70 and hsc70 are essentially interchangeable for the purposes of this course. Removing tom40 - i can isolate the protein and get it to the receptor but it won"t go anywhere because the receptor won"t hand it off to anything. Pex proteins are involved in the process of importing proteins into the peroxisome and making the peroxisomes. Proteins with a carboxy terminal associate with pts1. Precursor membrane - comes from the endoplasmic reticulum. The signals that identify peroxisomes come after this structure. The result is a ghost peroxisome: pex3 mutants can"t form the precursor structure and the result is no organelle and all membrane associated proteins in the cytoplasm. In b. and c. , catalase is left outside the organelle and in the cytoplasm.
