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Lecture 14

Anthropology 2235A/B Lecture Notes - Lecture 14: Southern Blot, Restriction Enzyme, Restriction Fragment Length Polymorphism


Department
Anthropology
Course Code
ANTH 2235A/B
Professor
Eldon Molto
Lecture
14

Page:
of 1
Lecture 14 RFLP and Sir Alec Jefferys
1985: Sir Alec Jefferys invented Restriction Fragment Length
Polymorphism (RFLP) for identification purposes
o First use was actually on an immigration case but shortly thereafter
was used to solve the first homicide case in Britain
o Involves many tedious steps and requires relatively large amounts
of target DNA, often more than is found at crime scenes
o Utilizes electrophoresis to separate targeted fragments of DNA cut
into manageable portions that are shown as bar graphs
Procedures used in RFLP Analysis
o Steps involved in the RFLP procedure used by Jefferys:
1. Restriction enzyme cuts DNA
2. Fragments separated by electrophoresis
3. Southern blotting
4. Use of radioactive fluorescent gene probe to detect
fragments
5. If the segment is present it will show as a band of
radioactivity
Length Polymorphisms
o Most of the human genome is composed of the same DNA
o Individuals differ from loci because of the number of repeated
united called variable number tandem repeats (VNTRs)
o Called junk since they don’t make proteins