Anthropology 2235A/B Lecture Notes - Lecture 14: Southern Blot, Restriction Enzyme, Restriction Fragment Length Polymorphism
11 Jun 2012
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Lecture 14 rflp and sir alec jefferys. 1985: sir alec jefferys invented restriction fragment length. Polymorphism (rflp) for identification purposes: first use was actually on an immigration case but shortly thereafter was used to solve the first homicide case in britain. Involves many tedious steps and requires relatively large amounts of target dna, often more than is found at crime scenes: utilizes electrophoresis to separate targeted fragments of dna cut into manageable portions that are shown as bar graphs. Procedures used in rflp analysis: steps involved in the rflp procedure used by jefferys: Use of radioactive fluorescent gene probe to detect fragments. If the segment is present it will show as a band of radioactivity. Length polymorphisms: most of the human genome is composed of the same dna.
