Biochemistry 2280A Lecture Notes - Cloning, Dna Profiling, Styrofoam
12 Dec 2012
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11/16/2012: one of the three stop codons. No trnas for stop codons: specific termination factors. Binding of release factor to the a site occurs during termination. Release factor adds water to the chain rather than another amino acid, which releases the peptide from the trna and terminates translation: catalysis reaction. Its intricate molecular interactions and importance make it a prime therapeutic target. See table 7. 3 do not memorize mechanisms but understand how they work. Assuming the dna is 3000 base pairs, how many molecules of template are added: one base pair has a mass of about 600 daltons (g/mole) 1 mole of a dna of 3000 bp has a mass of: 3000 bp x 600 g/bp = 18 x 105 (approx. 1 mole 2 x 106 , x mole 100 ng (1 x 10-7) X = 1 x 10-7 / 2 x 106 = 5 x 10-14. 5 x 10-14 x 6. 02 x 1023 (avogadro"s number) = 3 x 1010.
