Biochemistry 2280A Lecture Notes - Gram-Negative Bacteria, Contig, Oligonucleotide
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Sequencing genomes: craig ventor vs. francis collins (racing to sequence the genome, ended up work- ing together to get it done faster, the language of life: dna and the revolution in personalized medicine - fran- cis collins. Sequencing a genome involves: creating a genomic dna library, many independent sequencing reactions, aligning the independent sequences into a continuous sequence. Genomic library: definition: a collection of cloned dna fragments that represent all of the dna in an organism"s genome, each cloned dna is like a different book. Constructing a genomic library: figure 10. 11, human dna is cleaved with restriction nuclease to create millions of genomic. Dna fragments: fragments are inserted into plasmids using ligase, recombinants dna molecules are introduced (as plasmids) into bacteria. Sequence contig: a contiguous dna sequence representing a portion of the genome, assembled from many independent sequences - much bigger than 1 clone.