Biochemistry 2280A Lecture Notes - Lecture 19: Phosphodiester Bond, Nuclear Membrane, Nuclear Transport
Document Summary
7-methyguanosine is added to the 5" end of pre-mrna. Marks the 5" end of the mrna as being intact. Required for: mrna transport form the nucleus by aiding in the stabilizing it to protect it from degradation. Almost all eukaryotic mrnas end with a (~300 base) poly-a tail on the 3" end. After the stop codon, rna is cleaved ~30 bases following a aauuaa (polyadenylation signal) and a poly-a tail is added. Provides additional stability to mrna by reducing the effects of 3" exonucleases. Mark the 5" an 3" end of the mrna as being intact, when cell machinery sees this it can identify that it is a mature mrna than can be processed. Protect mrna from degradation of 5" and 3" exonucleases. Protein encoding sequences(exons) are interrupted by one or more noncoding sequences (introns) Introns are spliced out of the primary transcript to form mature mrna. Number of introns and exons can differ for genes.