Biochemistry 2280A Lecture Notes - Cloning, Base Pair, Palindromic Sequence

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 2280A
• Professor: Mel Usselman

Document Summary

Kpn-1 is another class of restriction enzymes that binds to the palindromic sequence 5" ggtacc 3" & 3". Ccatgg 5" (cutting between the cytosines) and leaves sticky ends with 3" overhangs. Ssp-1 is yet another class of restriction enzymes that binds to the palindromic sequence 5" aatatt 3" & 3" ttataa 5" (cutting directly in the middle) and leaves blunt ends. Therefore we can see that there are in fact three classes of restriction enzymes observable, those that leave: 5" overhangs, 3" overhangs and blunt ends. Some restriction enzymes cut identical sequences, but leave different overhanging ends. This is observed in bam-h1 (5" overhanging ends) and nla-4 (blunt ends). Bgl-2 and bam-h1 cut different sequences, but leave the same overhangs. If there is 1 nc-2 site every 1000 base pairs, a genome of. 3 billion basepairs will contain approximately 3,000,000 sites. Like any other enzyme each restriction enzyme has preferred conditions in which it functions.