Biochemistry 2280A Lecture Notes - Lecture 1: Prostate Cancer, Peptide, Mecha

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 2280A
• Professor: Bonnie Mac Lachlan

Document Summary

Rna polymerase core enzyme: multi-subunit enzyme and it is complex. 4 subunits in core enzyme: 2 alphas, a beta and a beta prime. This will make rna in a test-tube but will not recognize promoters that"s the jo(cid:271) of the sigma subunit. To get rna synthesis with promotor recognition you need both the core enzyme and a sigma subunit this is called the rna polymerase holoenzyme. This repetition of transcription is called the sigma cycle (because sigma helps to start transcription falls off a(cid:374)d (cid:396)ejoi(cid:374)s rnap afte(cid:396) it"s do(cid:374)e to sta(cid:396)t agai(cid:374)(cid:895) Some genes have better promoters (the -10s and -35s are not all the same) This is not dynamic though static regulation. There is more than 1 sigma factor, each recognises different promoter sequences. Most t(cid:396)a(cid:374)s(cid:272)(cid:396)i(cid:271)ed (cid:271)(cid:455) (cid:862) igma 70(cid:863) (cid:894)aka the housekeepi(cid:374)g sig(cid:373)a(cid:895) Gene specific regulatory proteins proteins that repress or activate transcription (euk and prok) Positive regulation factors activate transcription (catabolite activator protein)