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Lecture

Lecture 28 DNA cloning- BRANDL

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Department
Biochemistry
Course
Biochemistry 2280A
Professor
Chris Brandl
Semester
Fall

Description
DNA Ligase -DNA ligase will recongnize the nics and use energy of ATP. Ligase has harder time getting blunt ends together. -it is like glue that puts DNA back together -ligase requires phosphates at the end of the DNA. Steps in Cloning 1. Digest gene with restrictive enzyme. That will release our gene from the PCR product. Now we have sticky end one either side. 2. Plasmid is called the vector and the favourite gene is called the insert. 3. Purify the DNA – we do electrophoresis. 4. We do the ligation reaction, mix DNA ligae (with atp), vector and the insert into a test tube.
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