Biochemistry 2280A Lecture : Antibodies

40 views1 pages

orchidotter238

20 Dec 2011

School

Department

Course

Professor

For unlimited access to Class Notes, a Class+ subscription is required.

Document Summary

The antigen is the substance that causes the formation of the antibody bind together. If they aren"t similar they are variable regions allows the antibody to bind to different antigens-0hypervariable within variable are sensitive to amino acid changes. 2 light chains and 2 heavy chains that make up immunoglobulin each contains a variable region and a constant region. Igg is tetrameric (4 polypeptide chains) but there are 2 identical pairs. At the very tips of the light and heavy chain is the variable region bars represented hypervariable for slots of amino acids. Yhpervariable regions are critical of specificity for antigen binding site these amino acids will be very specific to the antigen. Both the heavy chain and the light chain contribute to antigen binding specificity. Often see immunoglobulin g written as a y because it takes that shape. Various domains are the loops folded region within a polypeptide.

Related Questions

Question II. A biochemist determines the amino acidsequence of the entire light chain from a purified immunoglobulinproduced by a patient whose genome was completely sequenced. Fromthis sequence this scientist is able to establish [complete thesentence and assess each subsequent statement (11-19) as eitherfactually correct (= âtrueâ) or incorrect (= âfalseâ)]:

*note that the question says that the entire light chain wasdetermined and the genome was completely sequenced*

11. the class of immunoglobulin.
12. the light chain isotype.
13. whether the B cell that produced this antibody received T-cellhelp.
14. the specific variable gene fragment used to produce thischain.
15. the antigen specificity of the immunoglobulin.
16. the number of VDJ rearrangements needed to produce this lightchain.
17. the most frequent amino acid present in this light chain.
18. the most frequent amino acid most likely to be present in theassociated heavy chain.
19. which antigens cannot react with this immunoglobulin.

jaderhinoceros645

PLEASE PROVIDE YOUR RATIONALE FOR EACH ANSWER.

Question II. A biochemist determines the amino acid sequence of the entire light chain from a purified immunoglobulin produced by a patient whose genome was completely sequenced. From this sequence this scientist is able to establish [complete the sentence and assess each subsequent statement (11-19) as either factually correct (= âtrueâ) or incorrect (= âfalseâ)]:

11. the class of immunoglobulin.
12. the light chain isotype.
13. whether the B cell that produced this antibody received T-cell help.
14. the specific variable gene fragment used to produce this chain.
15. the antigen specificity of the immunoglobulin.
16. the number of VDJ rearrangements needed to produce this light chain.
17. the most frequent amino acid present in this light chain.
18. the most frequent amino acid most likely to be present in the associated heavy chain.
19. which antigens cannot react with this immunoglobulin.

PLEASE PROVIDE YOUR RATIONALE FOR EACH ANSWER.

PLEASE ACTUALLY ANSWER TRUE OR FALSE FOR EACH QUESTION AS WELL

While comparing two different protein-sequencing methods requiring minute amounts of protein, you established the amino acid sequence of the entire light chain of a purified immunoglobulin. From this sequence you will be able to determine [complete the sentence and assess the resulting statements (1-6) as either factually correct (= true) or incorrect (= false)]:

1. the isotype of the immunoglobulin.

2. whether the B cell that produced this antibody received T-cell help.

3. the specific variable gene fragment used to produce this chain.

4. the antigen specificity of the immunoglobulin.

5. the approximated strength of the immunoglobulinâs binding to different Fc receptors.

6. the molecular weight of the post-translationally unmodified light chain.

fuchsiaelk366

Biochemistry 2280A Lecture : Antibodies

Document Summary

Get access

Related Documents

Biochemistry 2280A Lecture Notes - Lecture 7: Mast Cell, Peptide, Reaction Coordinate

Biochemistry 2280A Lecture Notes - Lecture 7: Activation Energy, Isomerization, Covalent Bond

Biochemistry 2280A Chapter Notes - Chapter 4: Hmg-Coa Reductase, X-Ray Crystallography, Rhodopsin

Related Questions