Biochemistry 2280A Lecture : Types of Column Chromatography

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orchidotter238

21 Dec 2011

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Gel filtration - if a protein is small it will get stuck in the holes and stick to the hole. If they are larger they will run through the column and they will come out earlier. The smaller proteins get held up in the beads and come out later. Ion exchange - bead is charged either plus or minus and the proteins will stick based on their charge. Results in a positively charged bead (cellulose) all the beads would have a positive charge because of the cellulose. So this would bind to negative proteins to get the protein off you put in more negative ions that bump it off the beads. Beads are positively charged and have attracted negatively charged ions. Add the protein comes down negatively charged and it binds to the bead. Bring multiple negatively charged ions and bump it off.

Related Questions

In lab this week, you will be given a sample containing a mixture of two proteins: cytochrome c (pI = 9.5) and ovalbumin (pI = 6.0). Cation exchange chromatography will be used to separate cytochrome c and ovalbumin. Which of the following statements below is CORRECT?

		At a pH of 6.5, cytochrome c will be positively charged and bind to the negatively charged cation exchange resin
		At a pH of 6.5, cytochrome c will be negatively charged and bind to the positively charged cation exchange resin
		At a pH of 6.5, ovalbumin will be positively charged and bind to the negatively charged cation exchange resin
		At a pH of 6.5, ovalbumin will be positively charged and bind to the positively charged cation exchange resin

During active transport in the neuron, the outside of the cell is _________________ and the inside is _______________.

positive, negative

more positive, less positive

more negative, less negative

negative, positive

In active transport, _____________ provides the energy to move molecules against their concentration gradient.

nucleus

potassium

sodium

adenosine triphosphate

________________ proteins can change shape when moving substances across the membrane.

Proteins that can be open or closed, based on different conditions, are called ___________.

bypass proteins

channel proteins

selective proteins

gated

Tunnels that allow charged ions to move easily across the plasma membrane without the use of energy are called ____________________.

channel proteins

glycolypipd proteins

phospholipid proteins

carbohydrate chains

____________ and ____________ make it easier for molecules to move across the plasma membrane.

small, negatively charged

small, positively charged

small, no charge

large, negatively charged

large, positively charged

Biochemistry 2280A Lecture : Types of Column Chromatography

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