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Biochemistry (847)
Eric Ball (118)
Lecture

Constructing a Genomic Library

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Department
Biochemistry
Course
Biochemistry 2280A
Professor
Eric Ball
Semester
Fall

Description
Constructing a Genomic Libraryisolated from many cellshuman DNA cleave with restriction nucleaseSequencing the Hinfluenza genome Gram Negative Bacteriagenome2 million bpThe H influenze genome is 2 million bp How many independent clones must be in the genomic library to cover the genome at least once assuming an average insert size of 2000bp1000 2 000 00020001000 would be the absolute minimum To be sure you have full coverage you would want an excess of several fold Creating the Genomic Librarystart with millions of cellsextra DNA sonicate it to get fragmentsDNA fragments of various sizes use DNA to make the genomic libraryagarose gel electrophoresis purify the DNA fragments of 2000bp DNA fragments 162 kb prepare a clone library 20 000 clones each represents an independent fragment of the genome
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