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Lecture

Restriction Mapping

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Department
Biochemistry
Course
Biochemistry 2280A
Professor
Eric Ball
Semester
Fall

Description
Restriction MappingA dna molecule can be defined by the number and positions of its restriction enzyme cut sites This is called restriction mapping dnafiguring out whereeach dna will give unique and distinct pattern If you have any dna of interest you can characterize that by defining position of restriction enzyme cut sites and how many it hasWhat would gel pattern of following 5000bp circular dna look like after digestion with EcoRI Sst1 and a double digest of EcoRI and SstlShown herewith EcoRI we get 3000 and the other side2000With SStl we only have one cute site and we get linearlized dna If we cut both together we get three fragments we get 3000 and we get 1500 and 500 restriction map for the dnaBigger bands are darker than smaller bands Thats cuz when u stain it eth br binds to dna on molar basismasswiseso mass for small is less than large so stains lighterWhat would the restriction map be for this 4000 bp linear molecule digest with EcoRi an dHindIILike any other enzyme EACH restriction enzyme ha
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