Biochemistry 2280A Lecture : Restriction Mapping
29 Dec 2011
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A dna molecule can be defined by the number and positions of its restriction enzyme cut sites. This is called restriction mapping dna figuring out where each dna will give unique and distinct pattern. If you have any dna of interest, you can characterize that by defining position of restriction enzyme cut sites and how many it has. What would gel pattern of following 5000bp circular dna look like after digestion with ecori, sst1 and a double digest of ecori and sstl. Shown here with ecori, we get 3000. and the other side 2000. With sstl, we only have one cute site and we get linearlized dna . If we cut both together, we get three fragments, we get 3000, and we get 1500 and: restriction map for the dna. That s cuz when u stain it, eth br binds to dna on molar basis masswise so mass for small is less than large so stains lighter.
