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Lecture

Topic 29

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Department
Biochemistry
Course
Biochemistry 2280A
Professor
Eric Ball
Semester
Fall

Description
Topic 29You have identified a human protein that will be if tremendous value in treating disease Your goal is to mass produce this proteinPHT EPO HGH Insulin Factor VIII Interleukins are examplesExpression using Recombinant DNA TechnologyMany proteins are difficult to obtain form their native source RDT can dramatically increase expression and facilitate purificationallows us to get a lot of the protein and purify it more easilyEcoli are the most commonly used organism for expressing YFP first try to express it in Ecoli if you want to purify your protein not all can be expressed but always first choice to try why ecoli grows quickly and inexpensively doubling time is less than 30 min high yields grow on simplistic means protein extracts are made easily cell walls can be easily brokengen
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