Biochemistry 2288A Lecture : Transgenic Organisms
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1 Jan 2012
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You are making a knockout mouse, after neomycin (positive) selection you treat the surviving cells with gancyclovir (negative selection) and more cells die. What does cell death after gancyclovir treatment indicate?
1. The targeting vector randomly inserted into the genome | ||
The targeting vector did not insert into the genome | ||
The targeting vector inserted into the genome by homologous recombination |