Biochemistry 2280A Lecture Notes - Precursor Mrna, Small Nuclear Rna, Nuclear Pore

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Formation of mrnas rna processing (eukaryotic cells): Primary rna transcript processed to become a translatable mrna exported out of the nucleus translation. 5"end of the message is capped with 7 methyl guanosine. Capping is required for rna export from the nucleus (marks the end of the mrna as being intact), aids in stabilizing the mrna from being degraded and acts as a translational signal. The addition of a long a-tract to the 3" end of the rna by enzymes downstream. The rna is first cleaved ~30 bases following an aauaaa sequence which is 3" to the coding region a string of a residues is then added. Enzymes recognize aauaaa sequence clips then polya tail is added. The polya tail helps protect the 3"end of the mrna from degradation: provides additional stability to the mrna by reducing effects of 3" endonucleases.

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